Quiescence is a reversible state in which the cell exits the cell cycle and suspends the majority of cellular processes, including transcription. One of the hallmarks of quiescence in any organism is extensive chromatin condensation. We have recently used a single-nucleosome resolution variation of Hi-C called Micro-C to determine that chromatin condensation in quiescent budding yeast results both from H4 tail-dependent local chromatin fiber folding and the formation of condensin-mediated chromatin loop domains that resemble mammalian topologically associating domains. Disruption of either structure leads to widespread loss of transcriptional repression. These structural features combined with the genetic tractability of yeast make quiescent yeast an excellent model for determining in vivo conformations and functions of chromatin at very high resolution. This system also provides exciting opportunities to test models of chromatin folding and loop extrusion in cells.